Anti-Human HSP90 Recombinant Antibody
CAT#: TAB-368CL
Recombinant Human monoclonal antibody expressed in CHO binding to Human HSP90. The anti-HSP90 antibody is capable of recognizing cell surface HSP90 with high sensitivity, and hence is capable of detecting cell surface HSP90 of living cell in FACS analysis. Because of its capability to specifically recognize cell surface HSP90 with high sensitivity, the antibody can be used, for example, for preparing the vaccine, for delivering a substance possessing a biological activity, for an adjuvant that enhances a function of a vaccine, a tumor antigen antibody or an anti-tumor cell antibody, and in a method of specifically detecting a cell expressing cell surface HSP90.
Tested Data
Gene Expression
WB
Figure 1 Anti-Human HSP90 Recombinant Antibody (TAB-368CL) in WB
Western blot analysis was performed by loading 0.1 µg (Lane 1), 0.3 µg(Lane 2), and 0.6 µg (Lane 3) recombinant human HSP90 onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-Human HSP90 Therapeutic Antibody (TAB-368CL) at a dilution of 1:500 overnight at 4°C on a rocking platform. Then probed with Goat anti-Mouse IgG HRP secondary antibody at a dilution of 1:2,000 for one hour. Chemiluminescent was detected.
DB
Figure 2 Anti-Human HSP90 Recombinant Antibody (TAB-368CL) in Dot Blot
Dot Blot analysis of TAB-368CL was performed by coating with human HSP90 antigen.
TAB-368CL incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
ELISA
Figure 3 Anti-Human HSP90 Recombinant Antibody (TAB-368CL) in ELISA
ELISA analysis of TAB-368CL was performed by coating with recombinant human HSP90 antigen. Then blocked with BSA, and incubated with Anti-Human HSP90 Therapeutic Antibody (TAB-368CL). The HRP-conjugated Goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2058/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2058/673_C12_1_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2058/667_C12_1_blue_red_green.jpg
Normal Tissue
Figure 4 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2058/ihc_selected.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2058/5779_A_5_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000080824-HSP90AA1
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Specifications
- Host Species
- Human
- Derivation
- Mouse
- Type
- Antibody
- Species Reactivity
- Human
- Related Disease
- Infectious Disease (unspecified)
Product Property
- Purity
- >95%, by SDS-PAGE, under reducing conditions
- Storage
- 4 °C, -20°C if preferred
Target
- Alternative Names
- Heat shock protein 90 homolog; HSP90
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(Creative Biolabs Cat# TAB-368CL, RRID: AB_3111921)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
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| TAB-111CT-S(P) | Human Anti-HSP90 Recombinant Antibody; scFv Fragment (TAB-111CT-S(P)) | Inhib, WB | Mouse scFv |
| TAB-111CT-F(E) | Human Anti-HSP90 Recombinant Antibody; Fab Fragment (TAB-111CT-F(E)) | Inhib, WB | Chimeric (mouse/human) Fab |
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| MHC-CN0017 | APC-A*02:01/Human HSP90 (ALLSSGFSL) MHC Tetramer | FCM | |
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